Here, we show that addition of LH in the FeTA system considerably enhances BPA minimum effective concentration in mouse and human but not in rat fetal testes. With the use of a culture system that we developed (fetal testis assay ), we previously showed that 10 nmol/L BPA reduces basal testosterone secretion of human fetal testis explants and that the susceptibility to BPA is at least 100-fold lower in rat and mouse fetal testes. Bisphenol S (BPS) and bisphenol F (BPF) are already or are planned to be used as BPA alternatives. In conclusion, these preliminary findings demonstrated that BDNF is synthesized and stored in the ovary of zebrafish, suggesting an involvement of this neurotrophin in oocyte development.īisphenol A (BPA) is a widely studied typical endocrine-disrupting chemical, and one of the major new issues is the safe replacement of this commonly used compound. Our results showed BDNF expression in follicle cell layer in later stage. The identification of the different stages of oocytes was carried out by morphological basis and BDNF was investigated by immunohistochemistry, in situ hybridization and qPCR. Thus, this study aims to investigate the presence and distribution of BDNF in the ovary of zebrafish, a teleost fish widely However, to date, there are no data concerning BDNF in teleost fish ovary. In the ovary, BDNF is involved in mammalian oocyte development, early embryo cleavage and blastocyst formation. However, BDNF, similar to other neurotrophins, acts on several peripheral It is largely known that BDNF in the nervous system promotes neuronal growth, differentiation, survival and synaptogenesis. Also, the primary amino acid sequences of zebrafish (Danio rerio) and human BDNF are 91% identical. It has been demonstrated that the DNA-deduced amino-acid sequence of the processed mature BDNF of the teleost fish Xiphophorus maculatum shows 90% identity with the mouse sequence.

BDNF has been highly conserved molecule during the vertebrate evolution. Pietro Cacialli1,2, Livia D’angelo1, Paolo De Girolamo1, Carla Lucini1, Elisabeth Pellegrini2, Olivier Kah2, Luciana Castaldo1ġDepartment of Veterinary Medicine and Animal Productions, University of Naples Federico II, via Veterinaria, 1, 80137 Napoli, ItalyĢTeam NEED, IRSET, IFR 140, Rennes, derived neurotrophic factor (BDNF) is a member of the neurotrophin family, whose other components are nerve growth factor (NGF), neurotrophin (NT) 3, NT 4/5 and NT 6/7.

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